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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Rad51tm1Hst
targeted mutation 1, Paul Hasty
MGI:1857587
Summary 2 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
Rad51tm1Hst/Rad51tm1Hst involves: 129S7/SvEvBrd * C57BL/6 MGI:2669562
cx2
Rad51tm1Hst/Rad51tm1Hst
Trp53tm1Brd/Trp53tm1Brd
involves: 129S7/SvEvBrd * C57BL/6 MGI:3795437


Genotype
MGI:2669562
hm1
Allelic
Composition
Rad51tm1Hst/Rad51tm1Hst
Genetic
Background
involves: 129S7/SvEvBrd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rad51tm1Hst mutation (0 available); any Rad51 mutation (16 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• embryos die between E5.5 to E7.5
• at E7.5, embryos are beginning to resorb

embryo
• mutant cells derived from the inner cell mass failed to proliferate in vitro
• growth arrest at the egg cylinder stage (E5.5)
• at E7.5, embryos are smaller than controls
• homozygous embryos are described as hypocellular with cuboidal cell morphology
• apoptotic cells are frequently observed in epiblasts of E7.5 mutant embryos
• amniotic cavity fails to develop

growth/size/body
• at E7.5, embryos are smaller than controls

cellular
• apoptotic cells are frequently observed in epiblasts of E7.5 mutant embryos
• trophoblast cells in culture are reduced in number and fewer cells attach to culture plates after exposure to gamma irradiation
• mutant cells derived from the inner cell mass failed to proliferate in vitro
• mutant embryos incorporate less BrdU at E5.5 and E6.5 indicated reduced cellular proliferation




Genotype
MGI:3795437
cx2
Allelic
Composition
Rad51tm1Hst/Rad51tm1Hst
Trp53tm1Brd/Trp53tm1Brd
Genetic
Background
involves: 129S7/SvEvBrd * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Rad51tm1Hst mutation (0 available); any Rad51 mutation (16 available)
Trp53tm1Brd mutation (6 available); any Trp53 mutation (144 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• double mutant embryos survive longer than Rad51 homozygous mice; embryos appear grossly normal at E7.5, but are smaller at E8.5 and do not develop further

cellular
• MEFs derived from double-homozygous mice do not proliferate in culture and exhibit characteristics of cells entering premature senescence





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last database update
05/17/2016
MGI 6.03
The Jackson Laboratory