Mouse Genome Informatics
hm1
    Rad51tm1Hst/Rad51tm1Hst
involves: 129S7/SvEvBrd * C57BL/6
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
mortality/aging
• embryos die between E5.5 to E7.5
• at E7.5, embryos are beginning to resorb

embryogenesis
• apoptotic cells are frequently observed in epiblasts of E7.5 mutant embryos
• mutant cells derived from the inner cell mass failed to proliferate in vitro
• at E7.5, embryos are smaller than controls
• homozygous embryos are described as hypocellular with cuboidal cell morphology
• amniotic cavity fails to develop
• growth arrest at the egg cylinder stage (E5.5)

growth/size
• at E7.5, embryos are smaller than controls

cellular
• apoptotic cells are frequently observed in epiblasts of E7.5 mutant embryos
• trophoblast cells in culture are reduced in number and fewer cells attach to culture plates after exposure to gamma irradiation
• mutant cells derived from the inner cell mass failed to proliferate in vitro
• mutant embryos incorporate less BrdU at E5.5 and E6.5 indicated reduced cellular proliferation


Mouse Genome Informatics
cx2
    Rad51tm1Hst/Rad51tm1Hst
Trp53tm1Brd/Trp53tm1Brd

involves: 129S7/SvEvBrd * C57BL/6
Key:
phenotype observed in females WTSI Wellcome Trust Sanger Institute
phenotype observed in males EuPh Europhenome
N normal phenotype
mortality/aging
• double mutant embryos survive longer than Rad51 homozygous mice; embryos appear grossly normal at E7.5, but are smaller at E8.5 and do not develop further

cellular
• MEFs derived from double-homozygous mice do not proliferate in culture and exhibit characteristics of cells entering premature senescence