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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Stat4tm1Gru
targeted mutation 1, Michael J Grusby
MGI:1857248
Summary 8 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
Stat4tm1Gru/Stat4tm1Gru C.129S2-Stat4tm1Gru MGI:3617977
hm2
Stat4tm1Gru/Stat4tm1Gru involves: 129S2/SvPas MGI:3851529
hm3
Stat4tm1Gru/Stat4tm1Gru involves: 129S2/SvPas * BALB/c MGI:3617974
hm4
Stat4tm1Gru/Stat4tm1Gru NOD.129S2-Stat4tm1Gru MGI:3617975
ht5
Stat4tm1Gru/Stat4+ NOD.129S2-Stat4tm1Gru MGI:3617976
ht6
Stat4ity14/Stat4tm1Gru involves: 129S1/Sv * 129S2/SvPas * 129X1/SvJ MGI:5578205
cx7
Stat4tm1Gru/Stat4tm1Gru
Tg(Ins2-NP)25-3Olds/0
involves: 129S2/SvPas * BALB/c * C57BL/6 MGI:3620118
cx8
Stat4tm1Gru/Stat4tm1Gru
Tg(Ins2-GP)34-20Olds/0
involves: 129S2/SvPas * BALB/c * C57BL/6 MGI:3620122


Genotype
MGI:3617977
hm1
Allelic
Composition
Stat4tm1Gru/Stat4tm1Gru
Genetic
Background
C.129S2-Stat4tm1Gru
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Stat4tm1Gru mutation (4 available); any Stat4 mutation (46 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• wild-type CD4+ T cells polarized under Th17 conditions can be converted to a Th1-like cells (i.e. they express IFN-gamma) by culturing with IL-12
• mutant polarized Th17 CD4+ T cells fail to convert to the Th1-like state in the presence of IL-12
• reduction in the serum level of IFNG are seen compared to levels in diabetic and non-diabetic NOD mice, but serum level is comparable to that in Stat4-null NOD mice
• reduction in the serum level of IL-2 is seen compared to level in diabetic and non-diabetic NOD mice, but serum level of IL-12 is comparable to that of Stat4-null NOD mice

homeostasis/metabolism
• reduction in the serum level of IFNG are seen compared to levels in diabetic and non-diabetic NOD mice, but serum level is comparable to that in Stat4-null NOD mice
• reduction in the serum level of IL-2 is seen compared to level in diabetic and non-diabetic NOD mice, but serum level of IL-12 is comparable to that of Stat4-null NOD mice

hematopoietic system
• wild-type CD4+ T cells polarized under Th17 conditions can be converted to a Th1-like cells (i.e. they express IFN-gamma) by culturing with IL-12
• mutant polarized Th17 CD4+ T cells fail to convert to the Th1-like state in the presence of IL-12

renal/urinary system
• onset of ischemia effects is delayed

Mouse Models of Human Disease
DO ID OMIM ID(s) Ref(s)
type 1 diabetes mellitus DOID:9744 OMIM:222100
J:106340




Genotype
MGI:3851529
hm2
Allelic
Composition
Stat4tm1Gru/Stat4tm1Gru
Genetic
Background
involves: 129S2/SvPas
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Stat4tm1Gru mutation (4 available); any Stat4 mutation (46 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• T cells from mice immunized with keyhole limpet hemocyanin (KLH) in CFA (conjugated Freund's adjuvant) produce less IL-4 and interferon gamma than wild-type in response to KLH restimulation; IL-17a levels are comparable to controls




Genotype
MGI:3617974
hm3
Allelic
Composition
Stat4tm1Gru/Stat4tm1Gru
Genetic
Background
involves: 129S2/SvPas * BALB/c
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Stat4tm1Gru mutation (4 available); any Stat4 mutation (46 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cellular
• activated T lymphocytes stimulated with IL-12 for 48 hours do not proliferate, whereas activated lymphocytes from control mice exhibit dose-dependent proliferation

mortality/aging
• Salmonella-infected mice exhibit increased mortality compared with similarly treated heterozygous or wild-type mice

immune system
• activated T lymphocytes stimulated with IL-12 for 48 hours do not proliferate, whereas activated lymphocytes from control mice exhibit dose-dependent proliferation
• IL-12 stimulated NK cells do not show enhanced cytotoxicity compared to unstimulated cells, while stimulated control cells show a significant enhancement (30% specific lysis compared to 10% in unstimulated control cells)
• cultured lymphocytes differentiated into Th1 cells by anti-CD3 or heat-killed Listeria monocytogenes produce less than 20% of the IFNG produced by control cells; lymphotoxin production also is significantly reduced compared to control cells
• significant levels of Th2 cytokines IL-5 and IL-10 are detected in supernatants from these cultured lymphocytes
• lymphocytes cultured in the presence of IL-4 and IFNG produce significantly higher levels of Th2 cytokines than control cells
• in Salmonella-infected mice
• following Il-2 stimulation for 24 hours, lymphocytes from mutants do not show increased IFNG levels compared to a 15-fold increase seen in activated cells from control mice
• Salmonella-infected mice exhibit increased mortality, increased organ bacterial burden, and decreased IFN-gamma levels compared with similarly treated heterozygous or wild-type mice
• Salmonella-infected mice exhibit increased mortality compared with similarly treated heterozygous or wild-type mice

hematopoietic system
• activated T lymphocytes stimulated with IL-12 for 48 hours do not proliferate, whereas activated lymphocytes from control mice exhibit dose-dependent proliferation
• IL-12 stimulated NK cells do not show enhanced cytotoxicity compared to unstimulated cells, while stimulated control cells show a significant enhancement (30% specific lysis compared to 10% in unstimulated control cells)
• cultured lymphocytes differentiated into Th1 cells by anti-CD3 or heat-killed Listeria monocytogenes produce less than 20% of the IFNG produced by control cells; lymphotoxin production also is significantly reduced compared to control cells
• significant levels of Th2 cytokines IL-5 and IL-10 are detected in supernatants from these cultured lymphocytes
• lymphocytes cultured in the presence of IL-4 and IFNG produce significantly higher levels of Th2 cytokines than control cells

homeostasis/metabolism
• in Salmonella-infected mice




Genotype
MGI:3617975
hm4
Allelic
Composition
Stat4tm1Gru/Stat4tm1Gru
Genetic
Background
NOD.129S2-Stat4tm1Gru
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Stat4tm1Gru mutation (4 available); any Stat4 mutation (46 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• reduction in the serum level of IFNG is seen compared to diabetic and non-diabetic NOD mice
• a reduction in the serum level of IL-2 is observed compared to diabetic and non-diabetic NOD mice
• no female mutants develop diabetes (blood glucose levels above 13.9 mmol/l for 3 readings) over a 10-month period compared with 74% of female wild-type Stat4 NOD mice, which is similar to female NOD mice (80%)
• noc cellular infiltration is observed at 40 weeks of age compared to massive and invasive cellular infiltration seen in diabetic NOD controls and heterozygous Stat4 NOD or wild-type Stat4 mice
• a significant reduction in the numbers of CD4+ and CD8+ cells in islets is observed compared to diabetic NOD mice

endocrine/exocrine glands
• well-preserved insulin-positive cells are seen in the islets of mutants whereas such cells are rare in Stat4 heterozygous and wild-type Stat4 NOD mice
• compared to NOD controls, islets in Stat4 null NOD mice show well preserved insulin secretion at basal glucose levels and in response to high glucose

homeostasis/metabolism
• compared to NOD controls, islets in Stat4 null NOD mice show well preserved insulin secretion at basal glucose levels and in response to high glucose
• reduction in the serum level of IFNG is seen compared to diabetic and non-diabetic NOD mice
• a reduction in the serum level of IL-2 is observed compared to diabetic and non-diabetic NOD mice

Mouse Models of Human Disease
DO ID OMIM ID(s) Ref(s)
NOT type 1 diabetes mellitus DOID:9744 OMIM:222100
J:90600




Genotype
MGI:3617976
ht5
Allelic
Composition
Stat4tm1Gru/Stat4+
Genetic
Background
NOD.129S2-Stat4tm1Gru
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Stat4tm1Gru mutation (4 available); any Stat4 mutation (46 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
homeostasis/metabolism
• a significant decrease in the incidence of diabetes (30%) is observed compared with wild-type Stat4 NOD mice (74%); diabetes is diagnosed when 3 blood glucose measures are equal to or higher than 13.9 mmol/l

Mouse Models of Human Disease
DO ID OMIM ID(s) Ref(s)
type 1 diabetes mellitus DOID:9744 OMIM:222100
J:90600




Genotype
MGI:5578205
ht6
Allelic
Composition
Stat4ity14/Stat4tm1Gru
Genetic
Background
involves: 129S1/Sv * 129S2/SvPas * 129X1/SvJ
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Stat4ity14 mutation (0 available); any Stat4 mutation (46 available)
Stat4tm1Gru mutation (4 available); any Stat4 mutation (46 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• mice infected with Salmonella Typhimurium exhibit increased lethality compared with wild-type mice

mortality/aging
• mice infected with Salmonella Typhimurium exhibit increased lethality compared with wild-type mice




Genotype
MGI:3620118
cx7
Allelic
Composition
Stat4tm1Gru/Stat4tm1Gru
Tg(Ins2-NP)25-3Olds/0
Genetic
Background
involves: 129S2/SvPas * BALB/c * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Stat4tm1Gru mutation (4 available); any Stat4 mutation (46 available)
Tg(Ins2-NP)25-3Olds mutation (3 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• mutants have a lower degree of insulitis
• a 5-fold reduction in LCMV-specific CTL precursors is seen after viral challenge with LCMV
• splenocytes are unresponsive to IL-12 stimulation and do not produce significant levels oi IFNgamma
• a significant reduction in CD4+ T cell-dependent (2 consecutive measures of blood glucose >250 mg/dl) diabetes incidence is observed in mutants (30%) versus controls (85%) when infected with LCMV to induce IDDM

endocrine/exocrine glands
• mutants have a lower degree of insulitis

hematopoietic system
• a 5-fold reduction in LCMV-specific CTL precursors is seen after viral challenge with LCMV

Mouse Models of Human Disease
DO ID OMIM ID(s) Ref(s)
type 1 diabetes mellitus DOID:9744 OMIM:222100
J:107047




Genotype
MGI:3620122
cx8
Allelic
Composition
Stat4tm1Gru/Stat4tm1Gru
Tg(Ins2-GP)34-20Olds/0
Genetic
Background
involves: 129S2/SvPas * BALB/c * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Stat4tm1Gru mutation (4 available); any Stat4 mutation (46 available)
Tg(Ins2-GP)34-20Olds mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• Stat4 deficiency does not affect the incidence of CD4+ T cell independent diabetes

Mouse Models of Human Disease
DO ID OMIM ID(s) Ref(s)
type 1 diabetes mellitus DOID:9744 OMIM:222100
J:107047





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last database update
01/12/2022
MGI 6.17
The Jackson Laboratory