Cdh5^em2Anpko
Endonuclease-mediated Allele Detail

Summary

• Symbol: Cdh5^em2Anpko
• Name: cadherin 5; endonuclease-mediated mutation 2, Andrew P Kowalczyk
• MGI ID: MGI:8437133
• Synonyms: VE-cad^GGG
• Gene: Cdh5 Location: Chr8:104828257-104871143 bp, + strand Genetic Position: Chr8, 53.04 cM
• Alliance: Cdh5^em2Anpko page

Mutation origin

• Strain of Origin: Not Applicable

Mutation description

• Allele Type: Endonuclease-mediated (Not Applicable)
• Mutation: Nucleotide substitutions
• Mutation details:

  Glycine codons 649 (GGC) and 650 and 651(GGT) were changed top alanine (GCA) (p.G649_G651delinsAAA) using an sgRNA and an ssODN template with CRISPR/Cas9 technology. The mutation abolishes binding of the encoded protein to the Ctnnd1 protein, which leads to microvascular hemorrhaging and decreased survival. (J:291325)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cdh5 Mutation: 56 strains or lines available

References

• Original: J:291325 Grimsley-Myers CM, et al., VE-cadherin endocytosis controls vascular integrity and patterning during development. J Cell Biol. 2020 May 4;219(5)
• All: 1 reference(s)