Sptan1^{em2(SPTAN1)Bay}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Sptan1^{em2(SPTAN1)Bay}
• Name: spectrin alpha, non-erythrocytic 1; endonuclease-mediated mutation 2, Baylor College of Medicine
• MGI ID: MGI:8435607
• Gene: Sptan1 Location: Chr2:29855572-29921463 bp, + strand Genetic Position: Chr2, 20.93 cM, cytoband B
• Alliance: Sptan1^{em2(SPTAN1)Bay} page

Mutation origin

• Strain of Origin: C57BL/6J
• Project Collection: CPMM

Mutation description

• Allele Type: Endonuclease-mediated (Conditional ready, Inserted expressed sequence)
• Mutation: Insertion
• Mutation details:

  Using CRISPR/Cas9 editing in mouse embryos, exons 53, 54, 55, 56, and part of exon 57 in Sptan1 were replaced with corresponding SPTAN1 human (Hs) exons 53-57. Exon 53 was inserted in the antisense orientation, additionally a Lox71 site was inserted upstream of the antisense human exon 53 and a reverse complement Lox66 site was inserted downstream of the antisense human exon 53. Prior to cre-mediated recombination, the allele functions as a knock-out first with splicing from exons 52 to 54 to create a premature stop. With cre-mediated recombination, the antisense exon containing the variant are rotated into the sense orientation for expression of the variant-containing humanized region. (J:358610)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sptan1 Mutation: 130 strains or lines available

References

• Original: J:358610 Baylor College of Medicine, Baylor College of Medicine Alleles. MGI Direct Data Submission. 2024
• All: 1 reference(s)