Gt(ROSA)26Sor^{tm2(CAG-DNTT)Fwa}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm2(CAG-DNTT)Fwa}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 2, Frederick W Alt
• MGI ID: MGI:8394039
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm2(CAG-DNTT)Fwa} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:389722
• Parent Cell Line: EF1 (ES Cell)
• Strain of Origin: 129S/SvEv x C57BL/6

Mutation description

• Allele Type: Targeted (Inserted expressed sequence)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm2(CAG-DNTT)Fwa} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  DNTT (1791)

  Dntt	(MGI:98659)

• Mutation details:

  Embryonic stem cells carrying the Igk mutant Igk^tm2.1Fwa allele were re-targeted to introduce a CAG-hTdT cassette to intron 1 of the mouse Gt(ROSA)26Sor gene, downstream of the Igk allele on Chromosome 6. Expression of the human terminal deoxynucleotidyl transferase (hTdT; formal gene symbol DNTT, DNA nucleotidylexotransferase) cDNA is driven by the CAG promotor during light chain rearrangement in pre-B cells. It adds random nucleotides to VH-D and D-JH junctions to form N regions, and promotes human-like diversification of the light chain cassette. (J:101977, J:389722)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1243 strains or lines available

References

• Original: J:389722 Tian M, et al., Mouse models with human antibody repertoires for inducing multiple lineages of HIV-1 broadly neutralizing antibodies. bioRxiv. 2026 June 30
• All: 2 reference(s)