Gt(ROSA)26Sor^{tm1(RIT1*)Abrg}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(RIT1*)Abrg}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Alice Berger
• MGI ID: MGI:8378167
• Synonyms: Rosa26-LSL-RIT1^M90I
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(RIT1*)Abrg} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:373435
• Parent Cell Line: CJ7 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, Humanized sequence, Inserted expressed sequence)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm1(RIT1*)Abrg} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  RIT1 (6016)

  Rit1	(MGI:108053)

• Mutation details:

  A floxed neomycin resistance (neo) cassette and STOP sequence, followed by a mutant human Ras-like without CAAX 1 (RIT1) cDNA sequence, an internal ribosome entry site (IRES), and an enhanced green fluorescent protein (EGFP) sequence were inserted into the Gt(ROSA)26Sor locus. The human RIT1 sequence contains an ATG to ATA nucleotide substitution that results in a methionine to isoleucine mutation at amino acid position 90 (M90I) associated with the promotion of lung tumorigenesis. (J:101977, J:373435)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1243 strains or lines available

References

• Original: J:373435 Rominger MC, et al., Mutant RIT1 cooperates with YAP to drive an EMT-like lung cancer state. Cell Rep. 2025 Sep 24;44(10):116185
• All: 2 reference(s)