Gt(ROSA)26Sor^{tm1(CAG-RUNX1*,-EGFP)Dez}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(CAG-RUNX1*,-EGFP)Dez}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Dong-Er Zhang
• MGI ID: MGI:8353621
• Synonyms: Rosa26-AE9a
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(CAG-RUNX1*,-EGFP)Dez} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:344189
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Targeted (Conditional ready, Epitope tag, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm1(CAG-RUNX1*,-EGFP)Dez} expresses 1 gene
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  RUNX1 (861)

  Runx1

  (MGI:99852)

• Mutation details:

  The allele was designed to introduce a CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG), a loxP-flanked STOP (PGK-Neo-PGKpA) cassette, an HA-tagged human runt related transcription factor 1 (RUNX1) sequence, harboring the AE9a mutation, followed by an FRT-flanked internal ribosome entry site (IRES) and enhanced green fluorescent protein (EGFP) cassette, and polyA signal, all inserted into the Gt(ROSA)26Sor locus on Chromosome 6. (J:101977, J:344189)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1243 strains or lines available

References

• Original: J:344189 Yan M, et al., Single-cell RNA sequencing of a new transgenic t(8;21) preleukemia mouse model reveals regulatory networks promoting leukemic transformation. Leukemia. 2024 Jan;38(1):31-44
• All: 2 reference(s)