Frrs1l^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Frrs1l^{tm1c(EUCOMM)Hmgu}
• Name: ferric-chelate reductase 1 like; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:8350131
• Gene: Frrs1l Location: Chr4:56957173-56990391 bp, - strand Genetic Position: Chr4, 31.66 cM
• Alliance: Frrs1l^{tm1c(EUCOMM)Hmgu} page
• IMPC: Frrs1l gene page

Mutation origin

• Mutant Cell Line: HEPD0701_4_B11
• Germline Transmission: Earliest citation of germline transmission: J:361968
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 56971950 of Chromosome 4 upstream of the critical exon 3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 3 at position 56971950. The critical exon 3 is thus flanked by loxP sites. A "conditional ready" (floxed) allele created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:361968)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Frrs1l Mutation: 25 strains or lines available

References

• Original: J:361968 Mary Lyon Centre at MRC Harwell, Direct data submission from Mary Lyon Centre at MRC Harwell. 2025
• All: 1 reference(s)