Ngf^{tm1.1(NGF*)Cat}
Targeted Allele Detail

Summary

• Symbol: Ngf^{tm1.1(NGF*)Cat}
• Name: nerve growth factor; targeted mutation 1.1, Antonio Cattaneo
• MGI ID: MGI:8314973
• Gene: Ngf Location: Chr3:102377235-102428329 bp, + strand Genetic Position: Chr3, 45.25 cM
• Alliance: Ngf^{tm1.1(NGF*)Cat} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:282017
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Humanized sequence, Inserted expressed sequence, Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details:

  The human NGF coding sequence with an arginine to tryptophan substitution at codon 100 (p.R100W) and loxP site flanked NeoR/KanR cassette and PGK promoter replaced the mouse Ngf coding sequence via homologous recombination. The neo/kana cassette was removed through subsequent Cre-mediated recombination. The C-to-T transition at position 661 resulting in the p.R100W mutation is seen in patients with Hereditary Sensory and Autonomic Neuropathy type 5 (HSAN5). (J:282017)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Ngf Mutation: 15 strains or lines available

References

• Original: J:282017 Testa G, et al., The NGF(R100W) Mutation Specifically Impairs Nociception without Affecting Cognitive Performance in a Mouse Model of Hereditary Sensory and Autonomic Neuropathy Type V. J Neurosci. 2019 Dec 4;39(49):9702-9715
• All: 1 reference(s)