Smad4^tm1.1Ics
Targeted Allele Detail

Summary

• Symbol: Smad4^tm1.1Ics
• Name: SMAD family member 4; targeted mutation 1.1, Mouse Clinical Institute
• MGI ID: MGI:8280977
• Gene: Smad4 Location: Chr18:73772080-73836851 bp, - strand Genetic Position: Chr18, 49.51 cM
• Alliance: Smad4^tm1.1Ics page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:82809
• Parent Cell Line: S3 (ES Cell)
• Strain of Origin: C57BL/6NCrl

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A loxP site was inserted into intron11 and a STOP cassette (containing 3x SV40 poly(A) signal sequences), an FRT site flanked neomycin resistance gene cassette, 3' sequence of intron 11 and a modified copy of exon 12 were inserted into exon 12 (ENSMUSE00000337424, Smad4-201, GRCm39) immediately after the stop codon. The modification entails changing isoleucine codon 499 (ATT) to valine (GTT) (c.1495A>G p.I499V). The neo cassette was removed through subsequent Flp-mediated recombination. This allele expresses the wild-type protein and only after Cre-mediated deletion of the endogenous exon 12 coding region will it express the mutated protein. (J:82809)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Smad4 Mutation: 47 strains or lines available

References

• Original: J:82809 European Mouse Mutant Archive, Information obtained from the European Mouse Mutant Archive (EMMA). Unpublished. 2003-2013
• All: 1 reference(s)