Gt(ROSA)26Sor^{tm1.1(CAG-TVA,-EGFP,-RABVgp4*)Velm}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1.1(CAG-TVA,-EGFP,-RABVgp4*)Velm}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1.1, Dmitry Velmeshev
• MGI ID: MGI:8275289
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1.1(CAG-TVA,-EGFP,-RABVgp4*)Velm} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:101977
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Reporter)
• Mutation: Insertion
• Mutation details:

  A CAG-loxP-Stop-loxP-TVA-P2A-EGFP-P2A-oG-attB-neomycin-attP cassette was inserted into the mouse Gt(ROSA)26Sor locus via homologous recombination. CAG is the CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter. TVA represents an avian-specific retroviral receptor required for infection by EnvA-pseudotyped rabies virus. EGFP represents the enhanced green fluorescent reporter. oG is the optimized rabies glycoprotein that supports trans-synaptic spread. The neo cassette was removed with the phiC31 recombinase. (J:101977)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1243 strains or lines available

References

• Original: J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017
• All: 1 reference(s)