Gt(ROSA)26Sor^{tm2(CAG-EGFP,-Mars1*L274G)Esm}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm2(CAG-EGFP,-Mars1*L274G)Esm}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Erin M Schuman
• MGI ID: MGI:8252910
• Synonyms: 3xMetRS*
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm2(CAG-EGFP,-Mars1*L274G)Esm} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:373040
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm2(CAG-EGFP,-Mars1*L274G)Esm} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Mars1 (MGI:1345633)

• Mutation details:

  The allele consists of the CAG, floxed STOP-MetRS(L274G)-T2A-MetRS(L274G)-P2A-MetRS(L274G)-E2A-EGFP (WPRE ) inserted into the mouse ROSA26 locus using recombination-mediated cassette exchange in embryonic stem cells. The vector is designed to contain a mutant F3 FRT site, a NEO selection cassette, CAG promoter, a floxed STOP cassette, triple repeat of the mouse methionine-tRNA synthetase (Mars1) with the L274G point mutation separated by T2A, P2A, followed by E2A peptide sequence (to mediate ribosomal skipping) followed by Green Fluorescent Protein coding sequence, WPRE and an FRT site. This vector was integrated into the Gt(ROSA)26Sor locus by recombinase mediated cassette exchange (RMCE). The L274G mutation allows the binding of the methionine surrogate azidonorleucine (ANL) onto the methionine tRNA. ANL can be tagged for imaging or affinity purification. (J:373040)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1243 strains or lines available

Notes

Embryonic stem cells = A-A02? made for us by Taconic GmbH

References

• Original: J:373040 Alvarez-Pardo R, et al., Cell type-specific in vivo proteomes with a multicopy mutant methionyl tRNA synthetase mouse line. Lab Anim (NY). 2025 Sep;54(9):228-237
• All: 2 reference(s)