Rpl3l^em1Nove
Endonuclease-mediated Allele Detail

Summary

• Symbol: Rpl3l^em1Nove
• Name: ribosomal protein L3-like; endonuclease-mediated mutation 1, Eva Maria Novoa
• MGI ID: MGI:8246937
• Gene: Rpl3l Location: Chr17:24946800-24955117 bp, + strand Genetic Position: Chr17, 12.52 cM, cytoband A3.3
• Alliance: Rpl3l^em1Nove page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: CRISPR/cas9 mediated recombination using guide RNA targeting exon 5 created a 13 bp deletion. Quantitative RT-PCR, western blot, and immunoflourescence analyses confirmed the lack of expression in striated muscles from homozygous mice. (J:364838)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Rpl3l Mutation: 32 strains or lines available

References

• Original: J:364838 Milenkovic I, et al., Dynamic interplay between RPL3- and RPL3L-containing ribosomes modulates mitochondrial activity in the mammalian heart. Nucleic Acids Res. 2023 Jun 23;51(11):5301-5324
• All: 1 reference(s)