Gt(ROSA)26Sor^{tm27.1(CAG-EGFP,-HPV18E7)Ics}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm27.1(CAG-EGFP,-HPV18E7)Ics}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 27.1, Mouse Clinical Institute
• MGI ID: MGI:8238153
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm27.1(CAG-EGFP,-HPV18E7)Ics} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:82809
• Parent Cell Line: TB1 (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Mutation details: The following elements were inserted into an intron: the CAG promoter, a lox66 site, an FRT site flanked neomycin resistance gene cassette, inverted sequence coding for EGFP reporter, T2A self-cleaving peptide and HPV18 gene E7 (non-oncogenic antigen), SV40 poly(A) signal sequence, and an inverted lox71 site. The neo cassette was removed through subsequent Flp-mediated recombination. Only after Cre-mediated recombination will the EGFP-T2A-HPV18E7 sequence be flipped and expressed. (J:82809)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1210 strains or lines available

References

• Original: J:82809 European Mouse Mutant Archive, Information obtained from the European Mouse Mutant Archive (EMMA). Unpublished. 2003-2013
• All: 1 reference(s)