Gt(ROSA)26Sor^{tm22(CAG-DCLRE1C,-EGFP)Ics}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm22(CAG-DCLRE1C,-EGFP)Ics}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 22, Mouse Clinical Institute
• MGI ID: MGI:8238124
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm22(CAG-DCLRE1C,-EGFP)Ics} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:371088
• Parent Cell Line: BD10 (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Conditional ready, Epitope tag, Humanized sequence, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Mutation details: The following elements were inserted into an intron: the CAG promoter, a conditional STOP cassette (loxP site flanked cassette consisting of PGK promoter, neomycin resistance gene, PGK and 3x SV40 poly(A) signal sequences), followed by human DCLRE1C cDNA with in-frame sequence coding for a C-terminal cMyc tag, an FRT site, IRES sequence, EGFP reporter gene cDNA, a second FRT site and bovine GH poly(A) signal sequence. The inserted genes will only be expressed after Cre-mediated removal of the STOP cassette. (J:371088)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1211 strains or lines available

References

• Original: J:371088 Rivera-Munoz P, et al., Lymphopoiesis in transgenic mice over-expressing Artemis. Gene Ther. 2016 Feb;23(2):176-86
• All: 1 reference(s)