Bcl2l1^tm2.1Ics
Targeted Allele Detail

Summary

• Symbol: Bcl2l1^tm2.1Ics
• Name: BCL2-like 1; targeted mutation 2.1, Mouse Clinical Institute
• MGI ID: MGI:8238071
• Synonyms: Mt-xL
• Gene: Bcl2l1 Location: Chr2:152622588-152673648 bp, - strand Genetic Position: Chr2, 75.41 cM, cytoband H1
• Alliance: Bcl2l1^tm2.1Ics page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:371009
• Parent Cell Line: S3 (ES Cell)
• Strain of Origin: C57BL/6NCrl

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A loxP site was inserted into intron 2 and 3x poly(A) signal sequence was inserted into exon 3 (Bcl2l1-201, GRCm39) immediately downstream of the stop codon, followed by an FRT site flanked neomycin resistance gene cassette and a second loxP site. Downstream, 298bp of intron 2 and a modified exon 3 (where the sequence coding for the C-terminal transmembrane (TM) motif was replaced with sequence coding for the Acta1 mitochondrial targeting (MITO) signal) were inserted. The neo cassette was removed through Flp-mediated recombination, leaving the endogenous exon 3 floxed. This allele expresses wildtype protein and only after Cre-mediated deletion of the endogenous exon 3 will it express the mitochondria-directed peptide. (J:371009)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Bcl2l1 Mutation: 110 strains or lines available

References

• Original: J:371009 Gadet R, et al., The endoplasmic reticulum pool of Bcl-xL prevents cell death through IP3R-dependent calcium release. Cell Death Discov. 2024 Aug 1;10(1):346
• All: 1 reference(s)