Gt(ROSA)26Sor^{em2(CAG-Syp*,-mCherry)Ddg}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{em2(CAG-Syp*,-mCherry)Ddg}
• Name: gene trap ROSA 26, Philippe Soriano; endonuclease-mediated mutation 2, David D Ginty
• MGI ID: MGI:8235815
• Synonyms: Rosa26^{FSF-LSL-SYP1-miniSOG-mcherry}
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{em2(CAG-Syp*,-mCherry)Ddg} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:362872
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Endonuclease-mediated (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details:

  A CAG-FRT-Stop (hGH pA, HSV TK pA)-FRT-loxP-Stop (SV40 pA x3)-loxP-rat Syp cDNA-miniSOG-T2A-mCherry cassette was targeted to intron 1 of the mouse Gt(ROSA)26Sor gene using CRISPR-based homologous recombination techniques. The Syp-miniSOG-T2A-mCherry cassette consists of cDNA encoding rat synaptophysin (Syp) fused to miniSOG and a Thosea asigna virus 2A (T2A) sequence, followed by in frame with mCherry at the 3' end. The miniSOG (mini singlet oxygen generator) component is a small (106-residue) green fluorescent flavoprotein that efficiently generates singlet oxygen when illuminated by blue light. (J:101977, J:362872)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1243 strains or lines available

References

• Original: J:362872 Zhang D, et al., C-LTMRs evoke wet dog shakes via the spinoparabrachial pathway. Science. 2024 Nov 8;386(6722):686-692
• All: 2 reference(s)