Gt(ROSA)26Sor^{tm1(Nfkb-dEGFP*)Kopf}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(Nfkb-dEGFP*)Kopf}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Manfred Kopf
• MGI ID: MGI:8226103
• Synonyms: Gt(ROSA)26Sor^{tm1(Nfkb-destEGFP)Kopf}, KappaBle
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(Nfkb-dEGFP*)Kopf} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:370035
• Parent Cell Line: LK-1/LK1 (ES Cell)
• Strain of Origin: C57BL/6NHsd

Mutation description

• Allele Type: Targeted (Reporter)
• Mutation: Insertion
• Mutation details:

  Eight copies of an octameric NF-B response element is placed upstream of a destabilized version of EGFP containing a C-terminal PEST domain (destEGFP), which considerably reduces the half-life of EGFP and the background fluorescence in the absence of an activating stimulus. This construct was inserted into the Gt(ROSA)26Sor locus. Mice carrying this allele report specific NF-kappaB transcriptional activity. (J:370035)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1243 strains or lines available

References

• Original: J:370035 Tortola L, et al., KappaBle fluorescent reporter mice enable low-background single-cell detection of NF-kappaB transcriptional activity in vivo. Mucosal Immunol. 2022 Apr;15(4):656-667
• All: 5 reference(s)