Kit^em1Lmjn
Endonuclease-mediated Allele Detail

Summary

• Symbol: Kit^em1Lmjn
• Name: Kit proto-oncogene receptor tyrosine kinase; endonuclease-mediated mutation 1, Lauryl Nutter
• MGI ID: MGI:8213677
• Gene: Kit Location: Chr5:75735647-75817382 bp, + strand Genetic Position: Chr5, 39.55 cM
• Alliance: Kit^em1Lmjn page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (No functional change, Reporter)
• Mutation: Insertion
• Mutation details: This allele was generated at The Centre for Phenogenomics. A single repair template containing P2A, muGFP, and an SV40 polyA signal was delivered by incubating embryos with recombinant adeno-assoicated virus followed by electroporating Cas9 ribonucleoprotein complexes with a guide RNA with the spacer sequence GCATCTTCGTGCACGAGCAG. This resulted in insertion of P2A-muGFP in-frame with KIT after Chr5:75815202 (GRCm39). Two silent changes at p.L973 and p.L974 (Chr5:75815184G>C and Chr5:75815187C (J:322048)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kit Mutation: 177 strains or lines available

References

• Original: J:322048 The Centre for Phenogenomics, Direct Data Submission for The Centre for Phenogenomics Alleles. MGI Direct Data Submission. 2022
• All: 1 reference(s)