Cd48^em1Lmjn
Endonuclease-mediated Allele Detail

Summary

• Symbol: Cd48^em1Lmjn
• Name: CD48 antigen; endonuclease-mediated mutation 1, Lauryl Nutter
• MGI ID: MGI:8213675
• Gene: Cd48 Location: Chr1:171509577-171532826 bp, + strand Genetic Position: Chr1, 79.54 cM
• Alliance: Cd48^em1Lmjn page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (No functional change, Reporter)
• Mutation: Insertion
• Mutation details: This allele was generated at The Centre for Phenogenomics. A single repair template containing P2A, mTagBFP2, and an SV40 polyA signal was delivered by incubating embryos with recombinant adeno-assoicated virus followed by electroporating Cas9 ribonucleoprotein complexes with a guide RNA with the spacer sequence GGGTGAGAAGAGTTCTTGTC. This resulted in insertion of P2A-mTagBFP2 in-frame with CD48 after Chr1:171532442 with a deletion of the Cd48 stop codon Chr1:171532443 to 171532445 (GRCm39). The resulting allele is predicted express mTagBFP2 under control of the Cd48 promoter while leaving CD48 expression and function intact. (J:322048)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cd48 Mutation: 22 strains or lines available

References

• Original: J:322048 The Centre for Phenogenomics, Direct Data Submission for The Centre for Phenogenomics Alleles. MGI Direct Data Submission. 2022
• All: 1 reference(s)