Mbd5^{tm1c(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Mbd5^{tm1c(EUCOMM)Wtsi}
• Name: methyl-CpG binding domain protein 5; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:8164041
• Gene: Mbd5 Location: Chr2:48839511-49209702 bp, + strand Genetic Position: Chr2, 28.43 cM, cytoband C1
• Alliance: Mbd5^{tm1c(EUCOMM)Wtsi} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:342743
• Parent Cell Line: JM8.F6 (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_gt2 cassette was inserted at position 49139705 of Chromosome 2 upstream of exon 4 (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin resistance fusion gene sequence followed by a loxP site. An additional loxP site is inserted downstream of exon 4 at position 49140526. Exon 4 is thus flanked by loxP sites.A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking exon 4. (J:342743)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mbd5 Mutation: 66 strains or lines available

References

• Original: J:342743 Meziane H, et al., Large-Scale Functional Assessment of Genes Involved in Rare Diseases with Intellectual Disabilities Unravels Unique Developmental and Behaviour Profiles in Mouse Models. Biomedicines. 2022 Dec 6;10(12)
• All: 1 reference(s)