Ascc3^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Ascc3^{tm1c(EUCOMM)Hmgu}
• Name: activating signal cointegrator 1 complex subunit 3; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:8164017
• Gene: Ascc3 Location: Chr10:50468756-50727300 bp, + strand Genetic Position: Chr10, 24.87 cM
• Alliance: Ascc3^{tm1c(EUCOMM)Hmgu} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:342743
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 50493349 of Chromosome 10 upstream of exon 4 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of exon 4 at position 50494603. Exon 4 is thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking exon 4. (J:342743)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ascc3 Mutation: 130 strains or lines available

References

• Original: J:342743 Meziane H, et al., Large-Scale Functional Assessment of Genes Involved in Rare Diseases with Intellectual Disabilities Unravels Unique Developmental and Behaviour Profiles in Mouse Models. Biomedicines. 2022 Dec 6;10(12)
• All: 1 reference(s)