Gt(ROSA)26Sor^{em1(CAG-Trim21)Lmeh}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{em1(CAG-Trim21)Lmeh}
• Name: gene trap ROSA 26, Philippe Soriano; endonuclease-mediated mutation 1, Lisa Mehlmann
• MGI ID: MGI:8163997
• Synonyms: HA-Trim21
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{em1(CAG-Trim21)Lmeh} page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Epitope tag, Inserted expressed sequence)
• Mutation: Insertion
• Gt(ROSA)26Sor^{em1(CAG-Trim21)Lmeh} expresses 1 gene
  Knock-in expresses:
  

  Organism	Expressed Gene	Note
  mouse	Trim21 (MGI:106657)

• Gt(ROSA)26Sor^{em1(CAG-Trim21)Lmeh} expression driven by 1 gene
  Knock-in expression driven by:
  

  Organism	Driver Gene	Note
  chicken	CAG

• Mutation details:

  The allele was generated by cloning an HA-Trim21 sequence into the GT(ROSA)26Sor locus (Ai9, Addgene 22799), replacing the tdTomato sequence. The final targeting construct consists of a CAG (CMV enhancer/chicken beta-actin) promoter, a loxP-flanked 3xSV40 polyadenylation (STOP) signal, an HA-tagged tripartite motif-containing 21 (Trim21) sequence, a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE), and a BGH polyA signal. (J:101977, J:360169)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1243 strains or lines available

References

• Original: J:360169 Mehlmann LM, et al., Generation and Characterization of a TRIM21 Overexpressing Mouse Line. Genesis. 2024 Oct;62(5):e23616
• All: 2 reference(s)