Actn2^em1Mbp
Endonuclease-mediated Allele Detail

Summary

• Symbol: Actn2^em1Mbp
• Name: actinin alpha 2; endonuclease-mediated mutation 1, Mouse Biology Program, UC Davis
• MGI ID: MGI:7867285
• Synonyms: Actn2^V809I
• Gene: Actn2 Location: Chr13:12284312-12355613 bp, - strand Genetic Position: Chr13, 4.54 cM
• Alliance: Actn2^em1Mbp page

Mutation origin

• Strain of Origin: C57BL/6NCrl

Mutation description

• Allele Type: Endonuclease-mediated (Not Specified)
• Mutation: Nucleotide substitutions
• Mutation details: The mouse V809I model was created using optimized CRISPR Cas9 knock-in (KI) technology utilizing ribonucleoprotein (RNP) in the presence of a synthetic single-strand DNA repair template harboring the desired KI. The KI nucleotide is 6 bp from the cleavage site; the engineered A is within the PAM and will protect the HDR derived locus from auto-cutting by Cas9. (J:94077)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Actn2 Mutation: 56 strains or lines available

References

• Original: J:94077 Mutant Mouse Regional Resource Centers, Information obtained from the Mutant Mouse Regional Resource Centers (MMRRC). Unpublished. 2004-2015
• All: 1 reference(s)