Tg(Tmc1-Tmc2/Tmc1*)1Ajg
Transgene Detail

Summary

• Symbol: Tg(Tmc1-Tmc2/Tmc1*)1Ajg
• Name: transgene insertion 1, Andrew J Griffith
• MGI ID: MGI:7865855
• Synonyms: Tg[P(Tmc1)::Tmc2]
• Transgene: Tg(Tmc1-Tmc2/Tmc1*)1Ajg Location: unknown
• Alliance: Tg(Tmc1-Tmc2/Tmc1*)1Ajg page

Transgene origin

• Strain of Origin: C57BL/6J

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Tmc1-Tmc2/Tmc1*)1Ajg expresses 2 genes
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Tmc1 (MGI:2151016)	delta exon 8 and 9
  mouse	Tmc2 (MGI:2151017)

• Tg(Tmc1-Tmc2/Tmc1*)1Ajg expression driven by 1 gene
  Transgene expression driven by:
  

  Organism	Driver Gene	Note
  mouse	Tmc1 (MGI:2151016)

• Mutation details: BAC clone MSMg01-526L10 (181,748 bp, Riken Bioresource Center DNA Bank, Tsukuba, Japan), encoding wild-type MSM/Ms mouse genomic Tmc1 (170,745 bp) was used to construct Tg[Tmc1deltaEx8_9], Tg[PTmc1::Tmc2] using the Counter-Selection BAC Modification Kit with Red/ET recombination technology (Gene Bridges, Heidelberg, Germany). To construct Tg[Tmc1deltaEx8_9], 2775 bp including exons 8 and 9 were deleted using the same method. To construct Tg[PTmc1::Tmc2], Tg[Tmc1deltaEx8_9] was modified to replace the translation initiation codon of Tmc1 with the Tmc2 cDNA (NM_138655.1) conjugated with the SV40 polyadenylation signal, respectively. (J:278471)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:278471 Nakanishi H, et al., Tmc2 expression partially restores auditory function in a mouse model of DFNB7/B11 deafness caused by loss of Tmc1 function. Sci Rep. 2018 Aug 14;8(1):12125
• All: 1 reference(s)