Lrrk2^tm1.1Dral
Targeted Allele Detail

Summary

• Symbol: Lrrk2^tm1.1Dral
• Name: leucine-rich repeat kinase 2; targeted mutation 1.1, Dario R Alessi
• MGI ID: MGI:7865614
• Gene: Lrrk2 Location: Chr15:91557378-91700323 bp, + strand Genetic Position: Chr15, 46.07 cM, cytoband F1
• Alliance: Lrrk2^tm1.1Dral page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:101977
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Nucleotide substitutions
• Mutation details:

  The allele was designed to incorporate a conditional R1441G point mutation into exon 31 of the leucine-rich repeat kinase 2 (Lrrk2) gene. The targeting vector, which was inserted into the endogenous Lrrk2 intron 30, consists of an initial loxP site, followed by an FRT-flanked neomycin cassette, exons31-51 Lrrk2 cDNA including the 3' untranslated region (UTR), an hGHpA (2.1 kb of the human growth hormone gene harboring the hGH polyadenylation signal), an F3-flanked puromycin cassette, and a second loxP site. Genomic exon 31 harboring the R1441G mutation is located immediately downstream of the second loxP site. FLP-recombinase removed the FRT-flanked neomycin cassette and the F3-flanked puromycin cassette. (J:101977)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Lrrk2 Mutation: 166 strains or lines available

References

• Original: J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017
• All: 1 reference(s)