Gt(ROSA)26Sor^tm1(MYC)Mro
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^tm1(MYC)Mro
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Silvia Marino
• MGI ID: MGI:7859517
• Synonyms: Gt(ROSA)26Sor^{tm(cMYC,-EGFP)Mro}
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^tm1(MYC)Mro page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:358615
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Mutation details: The Gateway Entry system was chosen to guarantee controlled and efficient monosite insertion of the c-MYC construct into the ubiquitously expressed Gt(ROSA)26Sor locus. The toxin-encoding ccdB gene was excised from the pENTR1A vector (Invitrogen) by BamH1/Xho1 restriction digest and a 1.5kb cDNA construct of human c-MYC was inserted into these sites of the pENTR1A vector. BamH1/Xho1 restriction digest showed correct insertion of the construct. Correct orientation of the insert was confirmed by DNA sequencing. The c-MYC construct was subsequently inserted into a targeting vector via in vitro recombination. Since the c-MYC construct in the pENTR1A vector is flanked by specific lambda phage integrase recognition sites (attL), the c-MYC construct could be efficiently transferred to a targeting vector carrying the corresponding heterotypic sites (attR). The targeting vector contains a 5 homology region to the mouse Gt(ROSA)26Sor genomic locus, a splice acceptor (SA) site, a PGK-neo-3x pA stop cassette flanked by loxP-sites (LSL), the c-MYC construct, an IRES-eGFP reporter gene, a 3 homology region to the mouse ROSA26 genomic sequence and a Diphteria Toxin A (DTA) selection cassette. (J:358615)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1095 strains or lines available

References

• Original: J:358615 Merve A, et al., c-MYC overexpression induces choroid plexus papillomas through a T-cell mediated inflammatory mechanism. Acta Neuropathol Commun. 2019 May 29;7(1):95
• All: 1 reference(s)