Rr381^em6(Rr96)Kap
Endonuclease-mediated Allele Detail

Summary

• Symbol: Rr381^em6(Rr96)Kap
• Name: regulatory region 381; endonuclease-mediated mutation 6, Dietmar J. Kappes
• MGI ID: MGI:7858944
• Synonyms: ThPOK^SIL.CD4Rx
• Gene: Rr381 Location: Chr3:89303454-89303824 bp Genetic Position: Chr3, Syntenic
• Alliance: Rr381^em6(Rr96)Kap page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Modified regulatory region)
• Mutations: Insertion, Intergenic deletion
• Mutation details: A 34 bp region in the Zbtb7b silencer, containing conserved Runx-binding motifs in addition to several predicted EGR, Ebox, and NFAT-binding sites, was targeted with a dsODN template using TALEN technology (ZFN target sequences (capitalized) ACCGCTACCCTAACCcataaCTGGAAGGGGTTTAG), resulting in its replacement with the equivalent 34 bp sequence from Cd4 silencer Rr96. (J:320244)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rr381 Mutation: 0 strains or lines available

References

• Original: J:320244 Basu J, et al., An autonomous TCR signal-sensing switch influences CD4/CD8 lineage choice in mice. Commun Biol. 2022 Jan 21;5(1):84
• All: 1 reference(s)