Tg(Ins2-TFRC/OVAL*)mT4Edp
Transgene Detail

Summary

• Symbol: Tg(Ins2-TFRC/OVAL*)mT4Edp
• Name: transgene insertion mT4, Ed Palmer
• MGI ID: MGI:7858863
• Synonyms: RIP-mT4
• Transgene: Tg(Ins2-TFRC/OVAL*)mT4Edp Location: unknown
• Alliance: Tg(Ins2-TFRC/OVAL*)mT4Edp page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Ins2-TFRC/OVAL*)mT4Edp expresses 2 genes
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  chicken

  OVAL (396058)

  human

  TFRC (7037)

  Tfrc	(MGI:98822)

• Tg(Ins2-TFRC/OVAL*)mT4Edp expression driven by 1 gene
  Transgene expression driven by:
  

  Organism

  Driver Gene

  Homolog in Mouse

  Note

  rat

  Ins2 (24506)

  Ins2	(MGI:96573)

• Mutation details: The transgene consists of the rat insulin promoter (Ins2) driving the first 118 residues of the human transferrin receptor (TFRC), which includes the cytoplasmic tail and signal/anchor domain, fused to mature chicken ovalbumin (OVAL), residues 139-387 coding for (SIINFEKL), which was mutated by site-directed mutagenesis to (SIITFEKL) resulting in a membrane-bound form of ovalbumin. (J:216727)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

References

• Original: J:216727 Koehli S, et al., Optimal T-cell receptor affinity for inducing autoimmunity. Proc Natl Acad Sci U S A. 2014 Dec 2;111(48):17248-53
• All: 1 reference(s)