Il33^tm1.2Bryc
Targeted Allele Detail

Summary

• Symbol: Il33^tm1.2Bryc
• Name: interleukin 33; targeted mutation 1.2, Paul Bryce
• MGI ID: MGI:7857161
• Synonyms: Il-33 KO
• Gene: Il33 Location: Chr19:29902514-29938118 bp, + strand Genetic Position: Chr19, 24.56 cM, cytoband C2
• Alliance: Il33^tm1.2Bryc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:361335
• Parent Cell Line: iTL IC1 (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion
• Mutation details: The targeting vector is designed to insert a loxP site upstream of exon 5, and an FRT-flanked and loxP-flanked neomycin resistance (neo) cassette downstream of exon 7 of the gene. An internal ribosome entry site (IRES) fused to an enhanced green fluorescent protein sequence was inserted into the 3 prime UTR. Flp-mediated recombination removed the FRT-flanked neo cassette leaving exons 5 through 7 floxed. Cre -mediated recombination in the germline removed exons 5-7. (J:361335)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Il33 Mutation: 32 strains or lines available

References

• Original: J:361335 Bisen S, et al., A neutrophil elastase-generated mature form of IL-33 is a potent regulator of endothelial cell activation and proliferative retinopathy. Exp Mol Med. 2024 Aug;56(8):1703-1716
• All: 1 reference(s)