Gt(ROSA)26Sor^{tm1(CAG-ChR2/tdTomato)Oki}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm1(CAG-ChR2/tdTomato)Oki}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Ole Kiehn
• MGI ID: MGI:7857119
• Synonyms: ROSA26-CAG-frt-STOP-frt-loxp-NLS/LacZ-loxP-ChR2-tdT
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm1(CAG-ChR2/tdTomato)Oki} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:82809
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Conditional ready, Reporter)
• Mutation: Insertion
• Mutation details: This RC::FLTG dual recombinase reporter construct contains a frt-flanked STOP cassette followed by a loxP-flanked NLS beta-gal::STOP cassette in front of ChR2-tdTomato. The ChR2-tdTomato contains a p2A self-cleaving peptide sequence between ChR2 and tdTomato, which leads to the expression of ChR2 and tdTomato as two separate proteins. This allele allows intersectional genetic optogenetic stimulation of different cell subpopulations defined by the overlap of FlipO and cre recombinase expression and can simultaneously be used for intersectional fluorescent labelling of cells since tdTomato is not bound to the membrane. (J:82809)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1046 strains or lines available

References

• Original: J:82809 European Mouse Mutant Archive, Information obtained from the European Mouse Mutant Archive (EMMA). Unpublished. 2003-2013
• All: 1 reference(s)