Rr544^em2Qli
Endonuclease-mediated Allele Detail

Summary

• Symbol: Rr544^em2Qli
• Name: regulatory region 544; endonuclease-mediated mutation 2, Qian Li
• MGI ID: MGI:7856730
• Synonyms: TAAR enhancer 1 & 2 double knockout
• Gene: Rr544 Location: Chr10:23806280-23806978 bp Genetic Position: Chr10, Syntenic
• Alliance: Rr544^em2Qli page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Modified regulatory region)
• Mutation: Intergenic deletion
• Mutation details: Taar enhancer TE1, located between Taar1 and Taar2, was targeted using sgRNAs (targeting TGGTTGTGAGTTGCTTGTGG, TCAGCCTGTTAATTACCTGA, AGAACTTTCAGAGAGTTCCC, GAACCCAGAACTGACTTTTG, TATTCTAGAAATACAGATGT and AGCATCCTGGAGGTGAAATG) with CRISPR/Cas9 technology, resulting in a 1205 bp deletion (GRCm39:chr10:23805948-23807152). This allele was created in zygotes carrying the Rr545^em1Qli TE2 deletion allele. This leads to the reduction in expression of most Taar genes without affecting olfactory gene expression. (J:322210)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rr544 Mutation: 0 strains or lines available

References

• Original: J:322210 Fei A, et al., Coordination of two enhancers drives expression of olfactory trace amine-associated receptors. Nat Commun. 2021 Jun 18;12(1):3798
• All: 1 reference(s)