Sec23a^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Sec23a^{tm1c(KOMP)Wtsi}
• Name: SEC23 homolog A, COPII coat complex component; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:7856721
• Synonyms: Sec32a^fl
• Gene: Sec23a Location: Chr12:59005170-59058803 bp, - strand Genetic Position: Chr12, 25.97 cM
• Alliance: Sec23a^{tm1c(KOMP)Wtsi} page
• IMPC: Sec23a gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:236027
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_gt2 cassette was inserted at position 59051784 of Chromosome 12 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT flanked lacZ/neomycin sequence followed by a loxP site. An additional loxP site is inserted downstream of the targeted exon(s) at position 59052582. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:236027)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sec23a Mutation: 47 strains or lines available

References

• Original: J:236027 Khoriaty R, et al., Pancreatic SEC23B deficiency is sufficient to explain the perinatal lethality of germline SEC23B deficiency in mice. Sci Rep. 2016 Jun 14;6:27802
• All: 2 reference(s)