Mettl3^tm1.1Jcbr
Targeted Allele Detail

Summary

• Symbol: Mettl3^tm1.1Jcbr
• Name: methyltransferase 3, N6-adenosine-methyltransferase complex catalytic subunit; targeted mutation 1.1, Jens C Bruning
• MGI ID: MGI:7856631
• Synonyms: Mettl3 f, Mettl3 flox
• Gene: Mettl3 Location: Chr14:52532298-52542585 bp, - strand Genetic Position: Chr14, 26.88 cM, cytoband C1
• Alliance: Mettl3^tm1.1Jcbr page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:280698
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying Mettl3^tm1Jcbr allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). The L1L2_Bact_P cassette was inserted at position 52535930 of Chromosome 14 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 4 at position 52536652. After flp recombination, the critical exon 4 is thus flanked by loxP sites. (J:280698)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mettl3 Mutation: 42 strains or lines available

References

• Original: J:280698 Cheng Y, et al., m(6)A RNA Methylation Maintains Hematopoietic Stem Cell Identity and Symmetric Commitment. Cell Rep. 2019 Aug 13;28(7):1703-1716.e6
• All: 1 reference(s)