Folh1^em1Ph
Endonuclease-mediated Allele Detail

Summary

• Symbol: Folh1^em1Ph
• Name: folate hydrolase 1; endonuclease-mediated mutation 1, Institute of Molecular Genetics, Praha
• MGI ID: MGI:7856624
• Gene: Folh1 Location: Chr7:86368185-86425151 bp, - strand Genetic Position: Chr7, 48.51 cM, cytoband D1-D2
• Alliance: Folh1^em1Ph page

Mutation origin

• Strain of Origin: C57BL/6NCrl

Mutation description

• Allele Type: Endonuclease-mediated (Not Specified)
• Mutation: Intragenic deletion
• Mutation details: TALENs were designed to target exon 11 of murine gene as follows: the left TALEN possessed the RVD sequence NG NG NN HD NI NI NN HD NG NN NN NN NI NG NN (targeted DNA sequence 5'-TTGCAAGCTGGGATG-3'), the right TALEN possessed the RVD sequence HD NI NN NG NI NN NI NI HD HD NI NI NN NI NI (targeted DNA sequence 5'-TTCTTGGTTCTACTG-3'). The TALENs were targeted to cleave within the chromosomal DNA sequence 5'CAGAAGAATTTGGCC-3'. One founder mouse carrying deletion of 6 bp (5'-CAGAAGGCC-3') was chosen to establish this allele. The deletion lies in the region encoding the active site of the protein. (J:82809)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Folh1 Mutation: 50 strains or lines available

References

• Original: J:82809 European Mouse Mutant Archive, Information obtained from the European Mouse Mutant Archive (EMMA). Unpublished. 2003-2013
• All: 1 reference(s)