Ikbke^{em1.2(EUCOMM)Wtsi}
Endonuclease-mediated Allele Detail

Summary

• Symbol: Ikbke^{em1.2(EUCOMM)Wtsi}
• Name: inhibitor of kappaB kinase epsilon; endonuclease-mediated mutation 1.2, Wellcome Trust Sanger Institute
• MGI ID: MGI:7852361
• Synonyms: Ikbke^{tm1c(EUCOMM)Wtsi}
• Gene: Ikbke Location: Chr1:131182337-131207339 bp, - strand Genetic Position: Chr1, 56.93 cM
• Alliance: Ikbke^{em1.2(EUCOMM)Wtsi} page
• IMPC: Ikbke gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:361968
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Endonuclease-mediated (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 131202021. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:361968)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Ikbke Mutation: 59 strains or lines available

References

• Original: J:361968 Mary Lyon Centre at MRC Harwell, Direct data submission from Mary Lyon Centre at MRC Harwell. 2025
• All: 1 reference(s)