Sap130^{tm1c(KOMP)Mbp}
Targeted Allele Detail

Summary

• Symbol: Sap130^{tm1c(KOMP)Mbp}
• Name: Sin3A associated protein; targeted mutation 1c, Mouse Biology Program, UC Davis
• MGI ID: MGI:7852339
• Synonyms: Sap30^flox
• Gene: Sap130 Location: Chr18:31767436-31856121 bp, + strand Genetic Position: Chr18, 17.81 cM, cytoband B2
• Alliance: Sap130^{tm1c(KOMP)Mbp} page
• IMPC: Sap130 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:360819
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 31780716 of Chromosome 18 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 31782954. The critical exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:360819)

Expression

In Mice Carrying this Mutation:

1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Sap130 Mutation: 50 strains or lines available

References

• Original: J:360819 Gabriel GC, et al., Mitotic block and epigenetic repression underlie neurodevelopmental defects and neurobehavioral deficits in congenital heart disease. Nat Commun. 2025 Jan 7;16(1):469
• All: 1 reference(s)