Harbi1^{tm1b(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Harbi1^{tm1b(EUCOMM)Hmgu}
• Name: harbinger transposase derived 1; targeted mutation 1b, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:7840188
• Gene: Harbi1 Location: Chr2:91541197-91551890 bp, + strand Genetic Position: Chr2, 50.63 cM
• Alliance: Harbi1^{tm1b(EUCOMM)Hmgu} page
• IMPC: Harbi1 gene page

Mutation origin

• Mutant Cell Line: HEPD0548_3_A02
• Germline Transmission: Earliest citation of germline transmission: J:361968
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Pgk_P cassette was inserted at position 110943342 of Chromosome 5 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the PGK promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 55949099. The critical exon(s) is/are thus flanked by loxP sites. A null/knockout allele was created by cre recombinase expression in mice carrying the tm1a allele to remove the neo selection cassette and loxP-flanked critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:361968)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Harbi1 Mutation: 22 strains or lines available

References

• Original: J:361968 Mary Lyon Centre at MRC Harwell, Direct data submission from Mary Lyon Centre at MRC Harwell. 2025
• All: 1 reference(s)