Ezh2^tm1.1Melb
Targeted Allele Detail

Summary

• Symbol: Ezh2^tm1.1Melb
• Name: enhancer of zeste 2 polycomb repressive complex 2 subunit; targeted mutation 1.1, Ari Melnick
• MGI ID: MGI:7798887
• Synonyms: Ezh2Y641F
• Gene: Ezh2 Location: Chr6:47507073-47572275 bp, - strand Genetic Position: Chr6, 22.92 cM, cytoband B
• Alliance: Ezh2^tm1.1Melb page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:234984
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Inserted expressed sequence)
• Mutation: Nucleotide substitutions
• Mutation details: A mini-gene comprising wild type exons 1620 and flanked by loxP sites was inserted into the endogenous intron 1516 of the gene followed by an frt-flanked neomycin selection cassette. A point mutation (TAC/TTC; aa, T > P) was engineered in exon 16 of the endogenous gene. The neomycin cassette was removed via FLP-mediated recombination in the germline. This allele expresses wild-type protein prior to cre-mediated recombination. Upon removal of the mini-gene, a mutant protein is expressed which contains the exon 16 mutation. (J:234984)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ezh2 Mutation: 71 strains or lines available

References

• Original: J:234984 Beguelin W, et al., EZH2 and BCL6 Cooperate to Assemble CBX8-BCOR Complex to Repress Bivalent Promoters, Mediate Germinal Center Formation and Lymphomagenesis. Cancer Cell. 2016 Aug 8;30(2):197-213
• All: 5 reference(s)