Cyp27b1^tm1.2Pike
Targeted Allele Detail

Summary

• Symbol: Cyp27b1^tm1.2Pike
• Name: cytochrome P450, family 27, subfamily b, polypeptide 1; targeted mutation 1.2, J Wesley Pike
• MGI ID: MGI:7791012
• Synonyms: C27KO
• Gene: Cyp27b1 Location: Chr10:126884119-126888875 bp, + strand Genetic Position: Chr10, 74.5 cM
• Alliance: Cyp27b1^tm1.2Pike page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:246808
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A loxP site was inserted into intron 6 and an FRT site flanked neomycin resistance gene cassette and a second loxP site downstream of the last exon. The neo cassette was removed through subsequent Flp-mediated recombination and exons 7-9 were deleted through subsequent Cre-mediated recombination. (J:246808)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Cyp27b1 Mutation: 33 strains or lines available

References

• Original: J:246808 Meyer MB, et al., A kidney-specific genetic control module in mice governs endocrine regulation of the cytochrome P450 gene Cyp27b1 essential for vitamin D3 activation. J Biol Chem. 2017 Oct 20;292(42):17541-17558
• All: 1 reference(s)