Gt(ROSA)26Sor^{tm15(CAG-PRKAR1A*,-EGFP)Ics}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm15(CAG-PRKAR1A*,-EGFP)Ics}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 15, Mouse Clinical Institute
• MGI ID: MGI:7788432
• Synonyms: Gt(ROSA)26Sortm15(CAG-PRKAR1a*,-eGFP)Ics
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm15(CAG-PRKAR1A*,-EGFP)Ics} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:82809
• Parent Cell Line: S3 (ES Cell)
• Strain of Origin: C57BL/6NCrl

Mutation description

• Allele Type: Targeted (Conditional ready, Humanized sequence, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Mutation details: The following elements were inserted into an intron: the CAG promoter, a conditional STOP cassette (loxP site flanked cassette consisting of PGK promoter, neomycin resistance gene, PGK and 3x SV40 p(A) signal sequences), followed by human PRKAR1A cDNA (variant ENST00000586397) where exon 6 was deleted, an FRT site, IRES sequence, the EGFP reporter gene, a second FRT site, and bovine GH poly(A) signal sequence. This allele only expresses PRKAR1A and EGFP after Cre-mediated removal of the stop cassette. (J:82809)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1095 strains or lines available

References

• Original: J:82809 European Mouse Mutant Archive, Information obtained from the European Mouse Mutant Archive (EMMA). Unpublished. 2003-2013
• All: 1 reference(s)