Gt(ROSA)26Sor^{tm11(Igk-V/Igh-V)Ics}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm11(Igk-V/Igh-V)Ics}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 11, Mouse Clinical Institute
• MGI ID: MGI:7788428
• Synonyms: Gt(ROSA)26Sortm11(LSL-anti-En1-2scFv-P2A-eGFP)Ics
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm11(Igk-V/Igh-V)Ics} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:82809
• Parent Cell Line: BD10 (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Mutation details: The following elements were inserted into an intron: an adenovirus splice acceptor site, a conditional STOP cassette (loxP site flanked cassette consisting of PGK promoter, neomycin resistance gene, PGK and 3x SV40 p(A) signal sequences), followed by the Igk signal peptide sequence, anti-En1 and En2 Igk-V/Igh-V single chain variable fragment (scfv) antibody cDNA with 6x Myc- and His-tag sequence in-frame, P2A self-cleaving sequence, the EGFP reporter gene, and SV40 poly(A) signal sequence. This allele only expresses the antibody and EGFP after Cre-mediated removal of the stop cassette. (J:82809)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1042 strains or lines available

References

• Original: J:82809 European Mouse Mutant Archive, Information obtained from the European Mouse Mutant Archive (EMMA). Unpublished. 2003-2013
• All: 1 reference(s)