Gt(ROSA)26Sor^{tm8(Igk-V/Igh-V)Ics}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm8(Igk-V/Igh-V)Ics}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 8, Mouse Clinical Institute
• MGI ID: MGI:7788217
• Synonyms: scFvOtx2^tg
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm8(Igk-V/Igh-V)Ics} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:291072
• Parent Cell Line: BD10 (ES Cell)
• Strain of Origin: C57BL/6NTac

Mutation description

• Allele Type: Targeted (Altered localization, Conditional ready, Epitope tag, Inserted expressed sequence, Reporter)
• Mutation: Insertion
• Mutation details: The following elements were inserted into an intron: an adenovirus splice acceptor site, a conditional STOP cassette (loxP site flanked cassette consisting of PGK promoter, neomycin resistance gene, PGK and 3x SV40 poly(A) signal sequences), followed by the Igk signal peptide sequence, anti-Otx2 Igk-V/Igh-V single chain variable fragment (scfv) antibody cDNA with 6x Myc- and His-tag sequence in-frame, P2A self-cleaving sequence, the EGFP reporter gene, and SV40 poly(A) signal sequence. This allele only expresses the antibody and EGFP after Cre-mediated removal of the STOP cassette. (J:291072)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1210 strains or lines available

References

• Original: J:291072 Bernard C, et al., A Mouse Model for Conditional Secretion of Specific Single-Chain Antibodies Provides Genetic Evidence for Regulation of Cortical Plasticity by a Non-cell Autonomous Homeoprotein Transcription Factor. PLoS Genet. 2016 May;12(5):e1006035
• All: 4 reference(s)