Smad4^tm1Mlind
Targeted Allele Detail

Summary

• Symbol: Smad4^tm1Mlind
• Name: SMAD family member 4; targeted mutation 1, Mark Lindsay
• MGI ID: MGI:7788216
• Gene: Smad4 Location: Chr18:73772080-73836851 bp, - strand Genetic Position: Chr18, 49.51 cM
• Alliance: Smad4^tm1Mlind page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:94077
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6N

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The allele was designed to incorporate a conditional I499V (ATT to GTT) point mutation into exon 12 of the SMAD family member 4 (Smad4) gene. The targeting vector consists of an initial loxP site immediately upstream of the endogenous splice acceptor site of intron 11, coding sequence of endogenous/wildtype exon 12 of Smad4, a SV40 polyA sequence, a neomycin (neo) cassette flanked by self-deletion anchor sites (SDA) and diphtheria toxin A (DTA), which used for negative selection, and a second loxP site. The I499V (ATT to GTT) point mutation in exon 12 is located immediately downstream of the second loxP site. (J:94077)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Smad4 Mutation: 46 strains or lines available

References

• Original: J:94077 Mutant Mouse Regional Resource Centers, Information obtained from the Mutant Mouse Regional Resource Centers (MMRRC). Unpublished. 2004-2015
• All: 1 reference(s)