Rrm1^tm1.1Chab
Targeted Allele Detail

Summary

• Symbol: Rrm1^tm1.1Chab
• Name: ribonucleotide reductase M1; targeted mutation 1.1, Andrei Chabes
• MGI ID: MGI:7785121
• Synonyms: Rrm1^tm1Ach, Rrm1^Y285A
• Gene: Rrm1 Location: Chr7:102090902-102118978 bp, + strand Genetic Position: Chr7, 54.72 cM
• Alliance: Rrm1^tm1.1Chab page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:358418
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: C57BL/6

Mutation description

• Allele Type: Targeted (Humanized sequence)
• Mutation: Nucleotide substitutions
• Mutation details: Exons 8-15 were replaced with a construct containing a TA to GC mutation in exon 9, resulting in tyrosine to alanine substitution at amino acid 285 (p.Y285A). In addition, a neomycin cassette consisting of FRT-PGK-gb2-Neo-loxP-FRT, was inserted in the intron between exons 8 and 9. The neomycin selection cassette was removed via flp-mediated recombination. The Y285 residue has been identified as mutated in human cancer samples, is the region encoding the allosteric specificity site, and reduces ribonucleotide reductase activity. (J:358418)

Tumor Data

List all tumor models in MMHCdb carrying Rrm1^tm1.1Chab

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rrm1 Mutation: 41 strains or lines available

References

• Original: J:358418 Tran P, et al., Altered dNTP pools accelerate tumor formation in mice. Nucleic Acids Res. 2024 Nov 11;52(20):12475-12486
• All: 1 reference(s)