Gt(ROSA)26Sor^{tm2.1(CAG-APOBEC1/YTHDF2*)Kmey}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm2.1(CAG-APOBEC1/YTHDF2*)Kmey}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 2.1, Kate D Meyer
• MGI ID: MGI:7783574
• Synonyms: APOBEC1-YTH^mut
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm2.1(CAG-APOBEC1/YTHDF2*)Kmey} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:358650
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Targeted (Inserted expressed sequence)
• Mutation: Insertion
• Gt(ROSA)26Sor^{tm2.1(CAG-APOBEC1/YTHDF2*)Kmey} expresses 2 genes
  Knock-in expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  APOBEC1 (339)

  Apobec1

  (MGI:103298)

  human

  YTHDF2 (51441)

  Ythdf2

  (MGI:2444233)

  Mutant YTHDF2

• Mutation details: The targeting construct consists of a CAG (CMV enhancer/chicken beta-actin) promoter, a loxP-flanked STOP cassette, a rat apolipoprotein B mRNA editing enzyme, catalytic polypeptide 1 (APOBEC1) sequence fused to a human mutant YTH N6-methyladenosine RNA binding protein 2 (YTHDF2) sequence, followed by an HA tag, a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE), a BGH polyA signal, and an attB/attP-flanked PGK-FRT-Neo-polyA cassette was inserted into the GT(ROSA)26Sor locus. FLP-recombinase removed the neo cassette. (J:101977, J:358650)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 1095 strains or lines available

References

• Original: J:358650 Tegowski M, et al., Single-cell m6A profiling in the mouse brain uncovers cell type-specific RNA methylomes and age-dependent differential methylation. 6A profiling in the mouse brain uncovers cell type-specific RNA methylomes and age-dependent differential methylation. Nat Neurosci. 2024
• All: 2 reference(s)