Spns1^{tm1b(EUCOMM)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Spns1^{tm1b(EUCOMM)Wtsi}
• Name: SPNS lysolipid transporter 1, lysophospholipid; targeted mutation 1b, Wellcome Trust Sanger Institute
• MGI ID: MGI:7783428
• Synonyms: Spns1KO-LacZ
• Gene: Spns1 Location: Chr7:125969232-125976622 bp, - strand Genetic Position: Chr7, 69.01 cM, cytoband F4
• Alliance: Spns1^{tm1b(EUCOMM)Wtsi} page
• IMPC: Spns1 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:358593
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The L1L2_Bact_P cassette was inserted at position 125974001 of Chromosome 7 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 125974818. The critical exon(s) is/are thus flanked by loxP sites. A null/knockout allele was created by cre recombinase expression in mice carrying the tm1a allele to remove the neo selection cassette and loxP-flanked critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml (J:358593)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Spns1 Mutation: 44 strains or lines available

References

• Original: J:358593 Beenken A, et al., Spns1 is an iron transporter essential for megalin-dependent endocytosis. Am J Physiol Renal Physiol. 2024 Nov 1;327(5):F775-F787
• All: 1 reference(s)