H2az2^tm1Ics
Targeted Allele Detail

Summary

• Symbol: H2az2^tm1Ics
• Name: H2A.Z histone variant 2; targeted mutation 1, Mouse Clinical Institute
• MGI ID: MGI:7780065
• Synonyms: H2afv^-
• Gene: H2az2 Location: Chr11:6377229-6394443 bp, - strand Genetic Position: Chr11, 4.01 cM
• Alliance: H2az2^tm1Ics page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:288671
• Parent Cell Line: S3 (ES Cell)
• Strain of Origin: C57BL/6NCrl

Mutation description

• Allele Type: Targeted (Conditional ready, Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: The L1L2_Bact_P cassette was inserted, in reverse (gene) orientation, at position 6377006 of Chromosome 11 downstream of exon 5 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 p(A) signal sequences, a second FRT site and a second loxP site. A third loxP site is inserted, in reverse (gene) orientation, into intron 3 at position 6379646. Exons 4-5 are thus flanked by loxP sites. A conditional-ready (floxed) allele can be created by flp recombinase expression in mice carrying this allele. Subsequent cre expression results in a knockout mouse. If cre expression occurs without flp expression, a reporter knockout mouse will be created. (J:288671)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any H2az2 Mutation: 25 strains or lines available

References

• Original: J:288671 Belotti E, et al., H2A.Z is dispensable for both basal and activated transcription in post-mitotic mouse muscles. Nucleic Acids Res. 2020 May 21;48(9):4601-4613
• All: 1 reference(s)