Gdf5^{tm1c(EUCOMM)Hmgu}
Targeted Allele Detail

Summary

• Symbol: Gdf5^{tm1c(EUCOMM)Hmgu}
• Name: growth differentiation factor 5; targeted mutation 1c, Helmholtz Zentrum Muenchen GmbH
• MGI ID: MGI:7779631
• Synonyms: Gdf5^flox
• Gene: Gdf5 Location: Chr2:155782943-155787204 bp, - strand Genetic Position: Chr2, 77.26 cM
• Alliance: Gdf5^{tm1c(EUCOMM)Hmgu} page
• IMPC: Gdf5 gene page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:358046
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: EUCOMM

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion
• Mutation details: The Ifitm2_intron_L1L2_GT2_LF2A_LacZ_BetactP_neo cassette was inserted at position 155786065 of Chromosome 2 (Build GRCm39) within an endogenous exon, creating an artificial intron that splits the original exon into two artificial exons, where the downstream artificial exon is critical. The cassette is composed of the intron of the Ifitm2 gene containing an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 155786800. The critical artificial exon(s) is/are thus flanked by loxP sites. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). Further information on targeting strategies used for this and other EUCOMMTOOLs alleles can be found at http://www.knockoutmouse.org/about/eucommtools/vectors.

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gdf5 Mutation: 30 strains or lines available

References

• Original: J:358046 Pregizer S, et al., A Novel Mouse Model to Elucidate the Role of Gdf5 in Postnatal Joints. Orthopedic Research Society Annual Meeting. 2018;:Abstract 0007
• All: 2 reference(s)