Ednrb^em1(rtTA)Rlb
Endonuclease-mediated Allele Detail

Summary

• Symbol: Ednrb^em1(rtTA)Rlb
• Name: endothelin receptor type B; endonuclease-mediated mutation 1, Robin Lovell-Badge
• MGI ID: MGI:7767936
• Synonyms: Ednrb^{em1(Ednrb,rtTA)Crick}, Ednrb^{em1(rtTA)Crick}
• Gene: Ednrb Location: Chr14:104052055-104081764 bp, - strand Genetic Position: Chr14, 53.05 cM
• Alliance: Ednrb^em1(rtTA)Rlb page

Mutation origin

• Strain of Origin: C57BL/6J

Mutation description

• Allele Type: Endonuclease-mediated (Transactivator)
• Mutation: Insertion
• Mutation details: The Ednrb-T2A-rTA strain was generated by the Genetic Modification Service at the Francis Crick Institute using CRISPR-Cas9 assisted targeting to insert a dox-inducible reverse transactivator into exon 8 of the Ednrb gene via a GSG and T2A peptide linker and followed by a stop codon. The donor template contained a 774bp insert of GSG, T2A and rtTA3, with 692bp of homology at the 5' end and 700bp homology at the 3' end. The AAV donor vector was synthesised and packaged into AAV serotype 1 by VectorBuilder. The guide sequence used was 5'-ATAAATACAGCTCGTCTTGA-3', that was synthesised as a synthetic guide RNA by IDTTM. (J:357896)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ednrb Mutation: 105 strains or lines available

References

• Original: J:357896 Sheridan D, et al., Gonadotrophs have a dual origin, with most derived from pituitary stem cells during minipuberty. bioRxiv. 2024
• All: 1 reference(s)